RESEARCH AREA A - INTERFACES AFFECTING AGING OF THE NERVOUS SYSTEM
Konstantin Sparrer / Alberto Catanese
Project A01
The impact of aging on innate immune defenses in the brain
Aging weakens innate immune defenses in the brain, shifting protective responses of microglia towards harmful, chronic inflammation. Using iPSC-derived CNS organoids enriched with young and aged primary microglia, this project uses neuron-tropic Herpes-Simplex-Virus-1 and microglia-tropic Zika virus infection as models. Integration of multi-omics data coupled to CRISPR validation will identify age-dependent alterations. The goal is to uncover the underlying mechanisms of age-associated innate immune dysfunction to help design strategies to restore protection and limit neuroinflammation.
Birgit Liss
Project A02
Activity-dependent neuronal Ca2+ signaling and its role in aging and Parkinson’s disease
We aim to define cell-specific mechanisms that result in differential neuronal vulnerabilities to known global Parkinson’s Diseases (PD) stressors, to provide novel targets for neuroprotective PD-therapy. Particularly, individual neuronal electrical activity patterns, voltage gated Ca2+ channels (Cav), and associated Ca2+ signalling emerged as such intrinsic factors. Cav received particular attention, as epidemiological studies consistently indicate that for unclear reasons, patients treated for hypertension with Cav inhibitors have a lower risk of developing PD later in life.
Karin Danzer
Project A03
Aging as the central driver of a-synuclein oligomer associated pathology in Parkinson’s disease
Aging is both necessary and sufficient for PD development, while α-syn oligomers on their own are necessary but not sufficient. Karin Danzer will study how age-related alterations shape α-syn–driven pathology by integrating pharmacological, molecular, and cellular approaches across mouse and human systems. Focusing on α-syn–induced astrocyte and microglia senescence and PBMC-derived glia exposed to α-syn–positive PD cerebral fluid, this work will reveal how glial aging and α-syn pathology cooperate eventually creating a permissive environment necessary for clinical symptoms.
Leda Dimou
Project A04
Manipulation of glial cells to improve physiological aging of the brain
Aging impairs myelin integrity, NG2-glia differentiation, and microglial myelin clearance. The myelin-demanding white matter shows the strongest decline in oligodendrogenesis with microgliosis and oligodendrocyte loss. We propose that intrinsic NG2-glia aging, defective microglial lipid handling, and altered myelin drive this failure. We aim to boost oligodendrogenesis or improve microglial clearance. Using in vitro assays, in vivo microglial depletion and repopulation, and hiPSC- derived NG2-glia, we seek targets that induce myelin repair and support brain function in aging.
Franz Oswald / Bernd Baumann
Project A05
Notch/NF-kappaB crosstalk critically regulates SASP development in brain inflammaging
Aberrant Notch/NF-kB crosstalk is a central regulatory hub in brain inflammaging affecting synaptic homeostasis and contributing to age-related cognitive decline. Using interaction fine-mapping and single molecule tracking, Franz Oswald and Bernd Baumann will define the specific interfaces underlying this multi-dimensional crosstalk to reveal new targets for selective therapeutic intervention. Their work will also examine the senomorphic/senolytic effects of Notch agonists to suppress detrimental SASP expression.
Sebastian Iben
Project A06
The accuracy of ribosomal protein synthesis- a critical parameter in human aging?
The accuracy of ribosomal protein synthesis correlates with longevity and healthspan in different species and is regulated during human aging. Sebastian Iben will investigate in which human tissues this regulation is of relevance and how disturbances are leading to aging-associated disease. Longevity interventions will be examined for their influence on ribosomal accuracy and brain organoids will be used for the analysis of the role of ribosomal errors in Alzheimer´s disease. These investigations will allow to develop interventional strategies.
RESEARCH AREA B - AGING AND THE IMMUNE SYSTEM
Mona Vogel / Hartmut Geiger
Project B01
Mechanisms of stem cell rejuvenation by attenuation of the activity of the small RhoGTPase Cdc42
Pharmacological reduction of the elevated activity of the small RhoGTPase Cdc42 with the small molecule CASIN rejuvenates hematopoietic stem cells (HSCs) and extends lifespan. Mona Vogel and Hartmut Geiger will define the mechanisms by which CASIN restores the function of aged HSCs. To this end, they will focus on Cdc42 effector proteins for which the interaction with Cdc42 is affected by CASIN and on transient metabolic changes initiated by CASIN. Identifying CASIN initiated rejuvenation pathways of HSCs will facilitate rational development of targeted interventions.
Melanie Scharpf
Project B03
Sex-specific molecular aging at the interface of DNA damage and inflammatory response and its impact on aging-associated disease
The DNA damage response (DDR), driving senescence and inflammation, changes sex-specifically during aging in human hematopoietic cells. Melanie Scharpf will elucidate how sex mechanistically mediates these aging-related differences in DDR and inflammatory response, determining the impact of age-related changes in sex chromosome-linked gene expression, sex hormone levels and nuclear integrity. Moreover, she will test if sex-specific and aging-related DDR mechanisms modulate the progression of cancer. This knowledge will be critical for the development of sex- and age-specific interventions.
Corinna Setz / Hassan Jumaa
Project B05
Memory B Cell Expansion and Antibody Shifts as Drivers of Inflammaging
We previously showed that naïve B cells decline with age, while memory B cells expand and produce TNF-α and IL-6. We will validate these findings in larger cohorts and extend them to Parkinson’s disease (PD). Moreover, we will examine how age-dependent shifts in IgM and IgG contribute to inflammation and autoimmunity. We will also address the role of the voltage-regulated calcium channels in aberrant B cell activation in PD. Elucidating how such age-related changes in B cells predispose to inflammaging will allow for targeted interventions in aging and chronic neurodegenerative disease.
Hartmut Geiger / Hans Kestler
Project B06
Towards cell-type specific aging models: niche dynamics and lineage decisions in hematopoiesis
Myeloid skewing is a well-described phenomenon of aging, also driven by aging of the bone marrow niche. Hans Kestler and Hartmut Geiger will generate computational multiscale models of specific cell entities and their interactions to experimentally dissect regulatory networks of hematopoietic stem cell aging. A particular focus will be on WNT signaling as prime candidate and systemic tipping points in the niche/stem cell interactions that can be experimentally tested.
Medhanie Mulaw
Project B07N
Deciphering the aging landscape across human tissues using large-scale single-cell sequencing and machine learning models
Cells do not age uniformly. How do cellular aging states interact and collectively lead to tissue failure and organismal aging? Medhanie Mulaw will investigate how different cell-intrinsic aging trajectories contribute to tissue and organismal decline. Using AI, machine learning, and scalable single-cell sequencing, he will map the aging landscape across four human tissues (brain, skin, bone marrow, and peripheral blood), discover new biomarkers, and identify key molecular drivers. This research will lay the groundwork for targeted interventions at both tissue and organismal levels.
RESEARCH AREA C - INTERFACES THAT MODULATE ORGAN AND ORGANISMAL AGING
Alexandra Herrmann / Frank Kirchhoff
Project C01
Interplay between HIV and amyloids in human aging
We found that amyloid fibrils associated with age-related neurological disorders, such as Alzheimer`s and Parkinson`s, enhance HIV infection, and that HIV Envelope fragments seed amyloid formation. HIV infection of brain organoids also increases cellular senescence, indicating a harmful virus-host circuit. In the next funding period, we will dissect the underlying mechanisms and define how HIV and amyloids synergistically amplify and accelerate neuroinflammatory complications.
Maja Funk / Karin Scharffetter-Kochanek
Project C02
Aging of barrier organs at cell interfaces: Targeting senescent cell clearance in skin and lung
Barrier organs such as the skin and lungs are constantly exposed to environmental stressors and rely on coordinated interaction between structural and immune cells. Aging disrupts this crosstalk, leading to impaired clearance of senescent cells and higher disease risk. Karin Scharffetter-Kochanek and Maja Funk will dissect how senescent structural cells impair aged NK cells and macrophages in skin and lung and identify ways to rejuvenate them to restore their clearance capacity. Ultimately, the organ-spanning project aims to enhance senescent cell clearance to improve barrier tissue function.
Daniel Sauter
Project C03
Aging at the interface of placental trophoblasts, NK cells and endogenous retroviruses
Preeclampsia (PE) is a life-threatening pregnancy disorder linked to accelerated aging and abnormal placental senescence. We hypothesize that aberrant senescence of placental trophoblast cells and NK cells drives impaired placental development in PE. Using patient samples and ex vivo models, we will define the underlying molecular mechanisms and evaluate senescence-modulating therapies. With our project, we ultimately aim to improve prediction and treatment of PE and related adverse pregnancy outcomes.
Gilbert Weidinger
Project C04
The interface of regeneration and aging: zebrafish regeneration requires elevated anti-aging mechanisms
Zebrafish regenerate their hearts via cardiomyocyte cell cycle re-entry. In period one, we found that regenerating cardiomyocytes face replication stress, which BMP signaling overcomes, a rescue mechanism that also functions in human cells. We will 1) identify druggable molecular mechanisms for BMP-mediated stress relief, 2) assess genomic stress in non-cardiomyocytes during heart regeneration and BMP's role, 3) Study DNA damage repair in osteoblast dedifferentiation. These studies lay the basis for translating BMP-mediated genomic stress attenuation into anti-aging therapies.
Jan Tuckermann / Christof Gebhardt
Project C05
Changing nuclear and cytoplasmic interfaces of the glucocorticoid receptor during aging
The glucocorticoid receptor (GR) differentially affects adipocytes and myeloid cells during aging, exhibiting a nuclear and cytoplasmic role shaping immune metabolism. Jan Tuckermann and Christof Gebhardt will dissect the cytoplasmic versus nuclear interfaces of GR by biophysical characterization of its compartment- and cofactor-dependent mechanisms, and by characterization of mutant mice lacking nuclear but retaining cytoplasmic GR functions, as well as of young and aged human PBMCs. PROTACs will mitigate adverse GC effects while enhancing beneficial immune-metabolic outcomes during aging.
Dhayana Dallmeier / Wolfgang Wagner
Project C06
Disentangling blood-based biomarkers for aging
Understanding key blood-based and epigenetic biomarkers can help us to disentangle aspects of aging, such as biological age and frailty. Dhayana Dallmeier and Wolfgang Wagner will develop reliable biomarkers of ageing and evaluate their aging trajectories, investigate epigenetic networks, and refine epigenetic clocks for clinical application. The aim is to optimize patient risk assessment and adjust treatments according to the individual ageing process. Additionally, the identified biomarkers and co-regulation networks of age-associated DNA methylation will be examined in mice.
PROJECT AREA Z - ADMINISTRATION AND CENTRAL PROJECT
Project Z01
Hartmut Geiger / Bettina Möhrle
Administration
The central administration project for the planned CRC will coordinate the CRC and all training activities and support mechanisms. Assistance in preparation and submission of manuscripts, continuous support of the CRC homepage, and the organization of (inter)national scientific meetings will be provided by this central project. Travel and publication costs will also be estimated and organized by this project. Furthermore, this project will act as a central platform for internal and external contact and outreach.
Project Z02
Dhayana Dallmeier, Michael Denkinger, Hartmut Geiger, Andreas Liebold, Gudrun Weinmayr, Karin Scharffetter-Kochanek, Hubert Schrezenmeier
Tissues and animals for aging research – an interdisciplinary approach
Z02 is a core platform supplying human blood cells, HSCs, MSCs, skin biopsies, and aged C57BL/6 mice to CRC projects. A major strength is access to primary tissues from older adults, mainly through the ActiFE study, which also integrates sample-derived data into its rich database. Additional human samples come from Dermatology, Cardiothoracic Surgery, and Transfusion Medicine. BAUM provides aged mice